Lead

Anti-Lead Monoclonal Antibody

ORDERING INFORMATION

Catalog No.: 60001 (clone Pb1).
Size: 0.5 ml ascites.

BACKGROUND

Exposure to potentially neurotoxic levels of lead occurs in approximately 9% of American children under 6 years of age, and many urban dwellings still contain dangerous levels of lead paint.

SPECIFICATION SUMMARY

Antigen: Lead conjugated to keyhole limpet hemocyanin (KLH) by proprietary chemistry.
Host Species: Mouse
Antibody Class: IgM
Preservatives: None. Available on request.

SPECIFICITY

This antibody detects lead in the ppb range. Indirect ELISA endpoint titer is >1: 100,000.

APPLICATIONS

Indirect ELISA: optimized for lead-RSA (rabbit serum albumin) on solid phase at 200ng/well. Ascites diluted 1:100,000 produces an O.D. signal of 0.5.
Competition ELISA: with lead-RSA on the solid phase at 200ng/well, 50% inhibition of antibody binding is achieved with Pb2+ at 800ng/well.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Not all applications have been investigated.

DILUTION INSTRUCTIONS

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE AND STABILITY

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

PRODUCT REFERENCE

Lin TJ and Chung MF 2008 Sensors 8: 582-593. Refers to product no. 60001.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.

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