LAMP1 Monoclonal Antibody
Catalog No.: 56270 (clone Ly1C6)
Format: 100ug in PBS, pH 7.4, purified by Protein G affinity chromatography, 0.09% sodium azide, 50% glycerol.
Lysosome-associated membrane proteins (LAMP1 and LAMP2) are major constituents of the lysosomal membrane. The structures of these two proteins are closely related with 37% sequence homology. Both are transmembrane glycoproteins localized primarily in lysosomes and late endosomes. Cell surface LAMP1 and LAMP2 promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium which suggests that they are involved in adhesion of PBMC at sites of inflammation.
Antigen: Rat liver lysosome membranes.
Host Species: Mouse
Antibody Class: IgG1
Specificity: This antibody recognizes human, rat, and hamster LAMP1.
Accession no.: NP_036989.1 Gene ID: 25328
Immunoblotting: Use at 1ug/ml. A band of ~120kDa is detected.
Immunocytochemistry: Use at 5-10ug/ml.
These are recommended concentrations. Enduser should determine optimal concentrations for their application.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
Lewis V et al. 1985. J Cell Biol 100: 1839-1847.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.