Kynurenic Acid ELISA Kit (ECP7886)
$718.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| 96 Tests | ELISA | Pan-Species |  |
Overview
Product Name Kynurenic Acid ELISA Kit (ECP7886)
Description Kynurenic Acid ELISA Kit (ECP7886) is a Competitive Inhibition ELISA for the quantitiation of Kynurenic Acid in a variety of aqueous sample types.
Target Kynurenic Acid
Species Reactivity Pan-Species
Assay Type Competitive Inhibition
Applications ELISA
Properties
Molecular Mass 189.17 g·mol−1
Background Minimum Level of Detection: 0.89 ng/mL
Dynamic range of quantitation: 2.47-200 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Dynamic range of quantitation: 2.47-200 ng/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other aqueous sample types.
Assay Resolution Quantitative
Components
| Reagents | Quantity |
| Kynurenic Acid Well Plate, Ready to Use (96 Wells) | 1 Each (8 x 12 Wells) |
| Adhesive Plate Seal | 4 Each |
| Kynurenic Acid Standard | 2 Vials |
| Standard Diluent | 1 x 20mL |
| Detection Reagent A | 1 Vial |
| Resuspension Solution A | 1 x 300uL |
| Diluent A | 1 x 12mL |
| Detection Reagent B | 1 x 120uL |
| Diluent B | 1 x 12mL |
| Stop Solution | 1 x 6mL |
| TMB Substrate | 1 x 9mL |
| 30X Wash Buffer | 1 x 20mL |
Specificity Information
Target ID Kynurenic Acid
Alternative Names 4-Hydroxyquinoline-2-carboxylic acid, 492-27-3, 13593-94-7, Kynurenate
CAS Number 492-27-3
PubChem ID 3845
Research Areas Biochemicals
Background Kynurenic acid is a quinolinemonocarboxylic acid that is quinoline-2-carboxylic acid substituted by a hydroxy group at C-4. It has a role as a G-protein-coupled receptor agonist, a NMDA receptor antagonist, a nicotinic antagonist, a neuroprotective agent, a human metabolite and a Saccharomyces cerevisiae metabolite. It is a monohydroxyquinoline and a quinolinemonocarboxylic acid. It is a conjugate acid of a kynurenate.
Application Images
Description Typical standard curve
Additional Information
Additional Information Assay Duration: Approximately 2 hours
Handling
Storage 4°C for 1 month. Recommended -20°C for long-term storage until indicated expiration date.