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Anti-KOS1 Antibody (28108)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ELISA Mouse
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SKU: 28108 Categories: , ,
Overview
Product Name Anti-KOS1 Antibody (28108)
Description Anti-Kos1 Rabbit Polyclonal Antibody
Target KOS1
Species Reactivity Mouse
Applications WB,ELISA
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to amino acids 421-434 (QNQVQSLPAGSAEP) of mouse Kos1 conjugated to KLH at the N-terminus.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes mouse Kos1 protein.
Target Name Non-receptor tyrosine-protein kinase TNK1
Target ID KOS1
Uniprot ID Q99ML2
Alternative Names EC 2.7.10.2, Kinase of embryonic stem cells
Gene Name Tnk1
Sequence Location Membrane, Cytoplasm
Biological Function May function in signaling pathways utilized broadly during fetal development and more selectively in adult tissues and in cells of the lymphohematopoietic system. Could specifically be involved in phospholipid signal transduction (By similarity). Involved in negative regulation of cell growth. Has tumor suppressor properties. Plays a negative regulatory role in the Ras-MAPK pathway. {PubMed:12789265, PubMed:18974114}.
Research Areas Neuroscience
Background Kinase of embryonic stem cells (Kos1) is a non-receptor protein tyrosine kinase (NRPTK) and a new member of the Ack family of NRPTKs. Kos1 is a ubiquitously expressed 47-kDa protein with autotyrosine kinase activity that is developmentally regulated during embryogenesis. Kos1 is also upregulated following IL3 withdrawal from factor-dependent murine NSF/N1.H7 cells that undergo apoptosis, suggesting a role in growth inhibition. Data support a negative regulatory role for Kos1 in regulating the Ras-Raf1-MAPK growth pathway by a mechanism that requires its autotyrosine kinase activity.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500 dilution. A band of approximately 47 kDa is detected.

Positive control: transfected cell lysate.
References & Data Sheet
References Hoare, K et al. (2003) Oncogene 22: 3562-3577.