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Anti-KLK11/Hippostatin Antibody (34008)

$400.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ELISA Human
SKU: 34008 Categories: , ,
Overview
Product Name Anti-KLK11/Hippostatin Antibody (34008)
Description Anti-Hippostatin/KLK11 Rabbit Polyclonal Antibody
Target KLK11/Hippostatin
Species Reactivity Human
Applications WB,ELISA
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to aa residues 68-82 (YIVHLGQHNLQKEEG) of human
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human hippostatin/KLK11 (35 kDa) which has 86% sequence homology with mouse and rat.
Target Name Kallikrein-11
Target ID KLK11/Hippostatin
Uniprot ID Q9UBX7
Alternative Names hK11, EC 3.4.21.-, Hippostasin, Serine protease 20, Trypsin-like protease [Cleaved into: Kallikrein-11 inactive chain 1; Kallikrein-11 inactive chain 2]
Gene Name KLK11
Sequence Location [Isoform 1]: Secreted.; [Isoform 2]: Golgi apparatus.
Biological Function Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. {PubMed:10872828, PubMed:16467084}.
Research Areas Cancer research
Background Hippostatin, also known as KLK11, is a kallikrein-like serine protease, which has two alternatively spliced isoforms, brain- type and prostate-type. Hippostatin plays a role in the prostate, including reproductive and/or tumorigenic functions. Elevated serum levels of KLK11 were found in 70% of women with ovarian cancer and in 60% of men with prostate cancer. Analysis of the KLK11 biomarker in serum may aid in the diagnosis and monitoring of ovarian and prostatic carcinoma.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting : use at 1:500 dilution.

Positive control: LNCaP cell lysate
References & Data Sheet
References Diamandis,E.P. et al. (2002) Cancer Res. 62: 295-300.
Nakamura,T et al. (2003) Prostate 54: 299-305.