Anti-Kir2.1 K+ Monoclonal Antibody
Catalog No.: 11531 (clone S112B/14)
Size: 100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. The Kir2.1 inward-rectifier potassium ion channel is encoded by the KCNJ2 gene. A defect in this gene is associated with Andersen-Tawil syndrome.
Antigen: Synthetic peptide corresponding to aa 390-411 (cytoplasmic C-terminus) of mouse Kir2.1 (accession number P35561). Host Species: Mouse
Antibody Class: IgG1
This antibody recognizes mouse and rat Kir2.1. It does not cross-react with Kir2.2 or Kir2.3.
Immunoblotting: use at 1-10ug/ml. A band of ~55kDa is detected.
Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml Immunofluorescence: use at 1-10ug/ml.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat brain lysate.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.