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Anti-KDEL Antibody (11076-200)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 200ug WB,ICC,ELISA All
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SKU: 11076-200 Categories: , ,
Overview
Product Name Anti-KDEL Antibody (11076-200)
Description Anti-KDEL Antibody Mouse Monoclonal Antibody
Target KDEL
Species Reactivity All
Applications WB,ICC,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 10C3
Isotype IgG2a
Immunogen Peptide corresponding to aa 649-654 of rat Grp78 conjugated to KLH.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.2, 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by affinity chromatography
Specificity Information
Target ID KDEL
Research Areas N
Background The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor which is a membrane protein localized in the Golgi apparatus.
Application Images
Additional Information
Handling
Storage Stable for at least one year at or below -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: Use at 4ug/ml. Reactivity with proteins corresponding to the apparent molecular mass of Grp78 and Grp94 in samples from mammals, birds, insects, and plants.

Immunohistochemistry: Use at 5- 10ug/ml.

These are recommended concentrations.

End users should determine optimal concentrations for their applications.
References & Data Sheet