Anti-KCNQ4 K+ Channel Antibody (11512)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF,IP,AM Human, Mouse, Rat
SKU: 11512 Categories: , ,
Overview
Product Name Anti-KCNQ4 K+ Channel Antibody (11512)
Description Anti-KCNQ4 K+ Channel Mouse Monoclonal Antibody
Target KCNQ4 K+ Channel
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,ICC/IF,IP,AM
Host Mouse
Clonality Monoclonal
Clone ID S43-6
Isotype IgG1
Immunogen Fusion protein aa 2-77 of human KCNQ4 (K+7.4, KvLQT4, accession number P56696).
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse and rat KCNQ4.
Target Name Potassium voltage-gated channel subfamily KQT member 4
Target ID KCNQ4 K+ Channel
Uniprot ID P56696
Alternative Names KQT-like 4, Potassium channel subunitα KvLQT4, Voltage-gated potassium channel subunit Kv7.4
Gene Name KCNQ4
Accession Number NP_004691.2
Sequence Location Basal cell membrane; Multi-pass membrane protein. Note=Situated at the basal membrane of cochlear outer hair cells.
Biological Function Probably important in the regulation of neuronal excitability. May underlie a potassium current involved in regulating the excitability of sensory cells of the cochlea. KCNQ4 channels are blocked by linopirdin, XE991 and bepridil, whereas clofilium is without significant effect. Muscarinic agonist oxotremorine-M strongly suppress KCNQ4 current in CHO cells in which cloned KCNQ4 channels were coexpressed with M1 muscarinic receptors.
Research Areas Ion Channels
Background Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. The protein coded for by the KCNQ4 gene forms a potassium channel that is thought to play a critical role in the regulation of neuronal excitability, particularly in sensory cells of the cochlea. The current generated by this channel is inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone N43/6 (11512). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody (11512) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) KCNQ4 Antibody (D) Composite.
Description Immunohistochemistry analysis using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone N43/6 (11512). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody (11512) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Description Immunohistochemistry analysis using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone N43/6 (11512). Tissue: frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody (11512) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Description Western Blot analysis of Rat tissue lysate showing detection of KCNQ4 protein using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone N43/6 (11512). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody (11512) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-10ug/mL. A band of ~77kDa is detected.Immunohistochemistry and

Immunocytochemistry: use at 0.1-1ug/mL.

Immunofluorescence: use at 1-10ug/mL

These are recommended concentrations;

Enduser should determine optimal concentrations for their applications.

Positive control: COS cell lysate transiently expressing KCNQ4.
References & Data Sheet