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Anti-JunB (Phospho-Ser259) Antibody (43027)

$355.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC Human, Mouse, Rat
SKU: 43027 Categories: ,
Overview
Product Name Anti-JunB (Phospho-Ser259) Antibody (43027)
Description Anti-JunB (Phospho-Ser259) Rabbit Polyclonal Antibody
Target JunB (Phospho-Ser259)
Species Reactivity Human, Mouse, Rat
Applications WB,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of Serine 259 (P-B-S(p)-P-I) derived from human JunB and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human, mouse, and rat JunB only when phosphorylated at serine 259.
Target Name Transcription factor JunB
Target ID JunB (Phospho-Ser259)
Uniprot ID P17275
Gene Name JUNB
Gene ID 165161
Post Translational Modification JunB (Phospho-Ser259)
Target Phospho-ser259
Sequence Location Nucleus.
Biological Function Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[GC]TCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to an AP-1 consensus sequence and its transcriptional activity (By similarity). {UniProtKB:P09450}.
Research Areas Phosphospecific Antibodies
Background Transcription factor JunB is a protein that in humans is encoded by the JUNB gene and is involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5′-TGA[CG]TCA-3′.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~43kDa is detected.

Immunohistochemistry: use at dilution of 1:50- 1:100.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications.
References & Data Sheet