Anti-JAB-1 Antibody (56210)

$503.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IP Human, Mouse
SKU: 56210 Categories: , ,
Overview
Product Name Anti-JAB-1 Antibody (56210)
Description Anti-JAB-1 Mouse Monoclonal Antibody
Target JAB-1
Species Reactivity Human, Mouse
Applications WB,IP
Host Mouse
Clonality Monoclonal
Clone ID 8H8.5
Isotype IgG2a
Immunogen Mouse JAB-1 gst-fusion protein (aa 1-334) expressed in E. coli.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by affinity on GST
Specificity Information
Specificity This antibody recognizes human and mouse JAB-1.
Target Name COP9 signalosome complex subunit 5
Target ID JAB-1
Uniprot ID Q92905
Alternative Names SGN5, Signalosome subunit 5, EC 3.4.-.-, Jun activation domain-binding protein 1
Gene Name COPS5
Sequence Location Cytoplasm, cytosol, Nucleus, Cytoplasm, perinuclear region, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle
Biological Function Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2. {PubMed:11285227, PubMed:11337588, PubMed:12628923, PubMed:12732143, PubMed:19214193, PubMed:20978819, PubMed:22609399, PubMed:9535219}.
Research Areas Neuroscience
Background JAB-1 is a 38 kD protein which was originally described as a coactivator for cJUN. It is present in a large protein complex called the “signalosome†which has been proposed to play a signaling role in mammalian cells. This protein complex appears to be analogous to the COP9 complex in plants which plays an important role in light-mediated plant development. Although the specific function of JAB-1 is unclear, it appears to be involved in targeting proteins for degradation by the proteasome.
Application Images
Description Part of a customer review using 56210
Description Detection of human Jab1 protein in HeLa whole cell lysate using monoclonal antibody 56210
Description Customer Review using 56210 - D10 T cells were lysed in IP buffer (20mM Tris-HCl, pH 7.5, 50mM NaCl, 50mM NaF, 10mM -glycerophosphate, 1% Triton X-100, 1mM PMSF).
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting and

Immunoprecipitation: use at 0.5-1 ug/mL.

Positive controls: 293 cells and recombinant fusion protein.
References & Data Sheet