Anti-IRAK (CT) Antibody
Catalog No.: 2426
Size: 100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chroma-tography.
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and key mediator of gene expression during immune and inflammatory responses. NF-κB activates numerous genes in response to extracellular stimuli, such as IL-1, TNFα, LPS, oxidative stress, and mitogens. NF-κB is associated with IκB in cytoplasm. After stimulation, 1κB is phosphorylated and dissociates from NF-κB. NF-κB enters cell nuclei where it activates an array of genes. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) mediates a signaling cascade leading to NF-κB activation. IRAK is associated with IL-1 receptor subunits IL-1RI and IL-1RacP and serves as a signaling molecule to mediate IL-1 responses. IRAK also mediates IL-18-induced NF-κB activation.
Antigen: Peptide corresponding the C- terminus of human IRAK.
Host Species: Rabbit
Preservatives: 0.02% sodium azide.
This antibody recognizes full-length human IRAK (80 kD).
Immunoblotting: use at 1:1,000-1:2,000 dilution.
Immunoprecipitation: use 2-4 ug antibody per sample.
Positive control: Whole cell lysate from HeLa cells or THP-1 cells.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.