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Anti-IP6K2 Antibody (11018)

$378.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB Human
SKU: 11018 Categories: , ,
Overview
Product Name Anti-IP6K2 Antibody (11018)
Description Anti-IP6K2 Mouse Monoclonal Antibody
Target IP6K2
Species Reactivity Human
Applications WB
Host Mouse
Clonality Monoclonal
Clone ID 4F10
Isotype IgG2b
Immunogen Full-length recombinant human IP6K2.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human IP6K2.
Target Name Inositol hexakisphosphate kinase 2
Target ID IP6K2
Uniprot ID Q9UHH9
Alternative Names InsP6 kinase 2, InsP6K2, EC 2.7.4.-, P(i-uptake stimulator, PiUS
Gene Name IP6K2
Gene ID 51447
Accession Number NP_001005909
Sequence Location Nucleus
Biological Function Converts inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). {PubMed:10574768, PubMed:30624931}.
Research Areas Apoptosis
Background Inositol hexakisphosphate kinase 2 (IP6K2) catalyzes the production of diphosphoinositol pentakisphosphate (IP7) and has a role as a proapoptotic gene sensitizing cancer cells to apoptosis by cell stressors and anticancer drugs all of which depend on IP6K2 catalytic activity. IP6K2 is located in the 3p21.31 chromosomal region, which often undergoes allele loss in a variety of human cancers. Some heat shock proteins, especially Hsp90, can be antiapoptotic and the targets of anticancer drugs. Hsp90 binds IP6K2 and inhibits its catalytic activity. Drugs and selective mutations that abolish HSP90-IP6K2 binding elicit activation of IP6K2, leading to cell death. Thus, the prosurvival actions of HSP90 reflect the inhibition of IP6K2, suggesting that selectively blocking this interaction could provide effective and safer modes of chemotherapy.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: A band of ~49 kDa is detected. User should determine optimal concentrations for their application.

Positive control: purified human IP6K2 or NIH-OVCAR-3 cells after treatment with interferon (IFN)-b.
References & Data Sheet
References Morrison, BH et al. 2002 Oncogene 21: 1882-1889.