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Anti-Interleukin-1 Receptor (IL-1R) Antibody (40101)

$391.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 200ug ELISA,IHC,RIA Human
SKU: 40101 Categories: , ,
Overview
Product Name Anti-Interleukin-1 Receptor (IL-1R) Antibody (40101)
Description Anti-Interleukin-1 receptor Mouse Monoclonal Antibody
Target Interleukin-1 Receptor (IL-1R)
Species Reactivity Human
Applications ELISA,IHC,RIA
Host Mouse
Clonality Monoclonal
Clone ID IL1-31-22.2.1
Isotype IgG2b
Immunogen The extracellular domain of the type I human IL-1 receptor (Genzyme Diagnostics).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity These antibodies react with cell surface and soluble human IL-1 receptor; Specific epitopes are unknown, but they do partially inhibit (30-50%) binding of one another to IL-1 receptor. They do not block binding of IL-1 to receptor, and they do not cross-react with receptors for human IL-2 or IL-4.
Target Name Interleukin-1 receptor type 1
Target ID Interleukin-1 Receptor (IL-1R)
Uniprot ID P14778
Alternative Names IL-1R-1, IL-1RT-1, IL-1RT1, EC 3.2.2.6, CD121 antigen-like family member A, Interleukin-1 receptorα, IL-1R-α, Interleukin-1 receptor type I, p80, CD antigen CD121a [Cleaved into: Interleukin-1 receptor type 1, membrane form
Gene Name IL1R1
Sequence Location Membrane, Cell membrane, Secreted
Biological Function Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Involved in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (PubMed:10653850). {PubMed:10653850, PubMed:10671496}.
Research Areas Growth Factors, Cytokines, Receptors
Application Images
Additional Information
Handling
Storage These antibodies are stable for at leastone (1) year at -20°C to -70 C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions ELISA: Can be used as capture reagents or second antibody at a concentration of 5 ug/ml

RIA: Concentrations of 20-30 ng/ml of these antibodies will bind radiolabeled IL-1 receptor in a radioimmunoassay format.

Immunohistochemistry: IL-1 receptor can be detected on cultured cells using antibody concentrations of 10-100 ug/ml.
References & Data Sheet