Interleukin-1 Receptor (IL-1R)

Interleukin-1 Receptor Monoclonal Antibodies

ORDERING INFORMATION

Catalog No.: 40101, 40102, 40103.
Size: 200 ug Protein G-purified antibody in PBS, pH 7.4

SPECIFICATION SUMMARY

Antigen: The extracellular domain of the type I human IL-1 receptor (Genzyme Diagnostics).
Host Species: Mouse.
Antibody Class: 40101 and 40103 – IgG2b; 40102 = IgG2a.
Stabilizers: None
Preservatives: None. Available on request.

SPECIFICITY

These antibodies react with cell surface and soluble human IL-1 receptor; Specific epitopes are unknown, but they do partially inhibit (30-50%) binding of one another to IL-1 receptor. They do not block binding of IL-1 to receptor, and they do not cross-react with receptors for human IL-2 or IL-4.

APPLICATIONS
ELISA: Can be used as capture reagents or second antibody at a concentration of 5 ug/ml.
RIA: Concentrations of 20-30 ng/ml of these antibodies will bind radiolabeled IL-1 receptor in a radioimmunoassay format.
Immunohistochemistry: IL-1 receptor can be detected on cultured cells using antibody concentrations of 10-100 ug/ml.

DILUTION INSTRUCTIONS

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE AND STABILITY

These antibodies are stable for at leastone (1) year at -20 C to -70 C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.