Insulin receptor (beta subunit)

Insulin Receptor Monoclonal Antibodies

ORDERING INFORMATION

Catalog Nos.: 50101, 50102, 50103, 50104.
Format: 100ug Protein G-purified antibody in PBS, pH 7.4.

SPECIFICATION SUMMARY

Antigen: IM-9 lymphocytes (50101, 50102, 50103) or human placental insulin receptor (50104).
Gene ID: 3643
Accession no.: P06213
Host Species: Mouse
Antibody Subtype: 50101 = IgG2a; 50102 and 50103 = IgG1; 50104 = IgG2b.
Specificity: All four clones recognize human insulin receptor; low-level cross-reactivity with bovine and rabbit insulin receptor.

					Conc. for half-maximal effect (nM)
Cat. No.	Subunit Specificity	% Inhibition of Insulin Binding		Biological Activity	Inhibition of Insulin Binding	Stimulation of Lipogenesis
		IM-9	Adipocytes
50101 (83-14)	α	80	90	Insulin-like	0.05	0.01
50102 (83-7)	?	0	10	Insulin-like	–	1.0
50103 (47-9)	α	92	98	Inhibits insulin	0.2	0.3
50104 (18-44)	β	35	40	Insulin-like	0.2	0.3

Cat. No.	Immunoprecipitation	Western Blot	Receptor Kinase Assay
50101 (83-14)	+++	–	Activates receptor kinase
50102 (83-7)	+	–	Activates receptor kinase
50103 (47-9)	–	–	Blocks activation by insulin
50104 (18-44)	+	+	Activates receptor kinase

 

DILUTION INSTRUCTIONS

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE AND STABILITY

These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

PRODUCT REFERENCES

Soos et al. (1986) Biochem J 235: 199.
Brindle et al. (1990) Biochem J 268: 615.
Prigent et al. (1990) J Biol Chem 265: 9970.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.

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