Insulin receptor (beta subunit)

Insulin Receptor Monoclonal Antibodies

ORDERING INFORMATION

Catalog No.: 50101, 50102, 50103, 50104.
Size: 100 ug Protein G-purified antibody in PBS, pH 7.

SPECIFICATION SUMMARY

Antigen: 50101, 50102, 50103: IM-9 These antibodies are stable for at least lymphocytes. 50104: human placental
Host Species: Mouse.
Antibody Class: 50101 = IgG2a; 50102, 50103 = IgG1; 50104 = IgG2b
Stabilizers: None
Preservatives: None. Available on request.

SPECIFICITY

All four clones recognize the human insulin receptor; low-level of cross-reactivity with bovine and rabbit insulin receptor.

Subunit Cat. No. Specificity	% Inhibition of Insulin Binding IM-9 Adipocytes	Biological Inhibition of Activity Insulin Binding	Stimulation of Lipogenesis
50101 (83-14) α	80 90	Insulin-like 0.05	0.01
50102 (83-7) ?	0 10	Insulin-like –	1.0
50103 (47-9) α	92 98	Inhibits insulin 0.2	–
50104 (18-44) β	35 40	Insulin-like 0.2	0.3

APPLICATIONS

Cat. No. 50101 (83-14)	Immunoprecipitation +++	Western Blot –	Receptor Kinase Assay Activates receptor kinase
50102 (83-7)	+	–	Activates receptor kinase
50103 (47-9)	–	–	Blocks activation by insulin
50104 (18-44)	+	+	Activates receptor kinase

DILUTION INSTRUCTIONS

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE AND STABILITY

These antibodies are stable for at least one (1) year at -20C to -70C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

REFERENCES

Soos et al. (1986) Biochem J 235: 199;
Brindle et al. (1990) Biochem J 268: 615;
Prigent et al. (1990) J Biol Chem 265; 9970;

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.

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