Anti-ILP-2 Antibody (11005)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF Human, Mouse, Rat
SKU: 11005 Categories: , ,
Overview
Product Name Anti-ILP-2 Antibody (11005)
Description Anti-ILP-2 Rabbit Polyclonal Antibody
Target ILP-2
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,ICC,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 2-13 of human ILP-2 (accession no. NP_203127).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat ILP-2 (33kDa).
Target Name Baculoviral IAP repeat-containing protein 8
Target ID ILP-2
Uniprot ID Q96P09
Alternative Names Inhibitor of apoptosis-like protein 2, IAP-like protein 2, ILP-2, Testis-specific inhibitor of apoptosis
Gene Name BIRC8
Gene ID 112401
Accession Number NP_203127
Sequence Location Cytoplasm
Biological Function Protects against apoptosis mediated by BAX.
Research Areas Apoptosis
Background Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family including IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibit specific proteases. A novel member in the IAP protein family has been identified and designated ILP-2 for IAP-like protein-2 (1). ILP-2 has high homology to ILP-1, but is encoded by a distinct gene. ILP-2, unlike ILP-1, has no inhibitory effect on Fas and TNF induced apoptosis, but potently inhibits apoptosis induced by overexpression of Bax or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts with the processed caspase- 9.
Application Images
Description Western blot analysis of ILP-2 expression in human HepG2 (lane 1) and MOLT4 (lane 2) cell lysates with ILP-2 antibody at 1 ug/mL.
Description Immunocytochemistry of ILP-2 in HepG2 cells with ILP-2 antibody at 10 ug/mL.
Description Immunofluorescence of ILP-2 in HepG2 cells with ILP-2 antibody at 20 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Immunocytochemistry: use at 10ug/mL.

Positive control: HepG2 or MOLT4 cell lysates.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
References & Data Sheet