Anti-IKKε (CT) Antibody
Catalog No.: 2329
Size: 100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chroma-tography.
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and key mediator of gene expression during immune and inflammatory responses. NF-κB activates numerous genes in response to extracellular stimuli, such as IL-1, TNFα, and LPS. NF-κB is associated with IκB in cytoplasm, which inhibits NF-κB activity. IκB is phos-phorylated by the IκB kinase (IKK) complex which contains IKKα, IKKβ, and IKKγ. A novel molecule in the IKK complex, designated IKKε, is required for the activation of NF-κB by PMA and T cell receptors but not TNFα and IL-1. IKKε is expressed in a variety of tissues and is inducible by TNFα, IL-1, and LPS.
Antigen: Peptide corresponding to aa 705-716 of human IKKε.
Host Species: Rabbit
Preservatives: 0.02% sodium azide.
This antibody recognizes human IKKε (80 kD). No cross-reactivity with IKKα, IKKβ or IKKγ.
Immunoblotting: use at 1:500-1:1,000 dilution.
Positive control: Whole cell lysate from Jurkat cells.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.