Anti-ICAD (NT) Antibody (2425)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Mouse
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Overview
Product Name Anti-ICAD (NT) Antibody (2425)
Description Anti-ICAD (NT) Rabbit Polyclonal Antibody
Target ICAD (NT)
Species Reactivity Mouse
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 2-21 at the N-terminus of mouse ICAD (accession no. O54786).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes non-cleaved (45kDa) and cleaved mouse ICAD.
Target Name DNA fragmentation factor subunitα
Target ID ICAD (NT)
Uniprot ID O54786
Alternative Names DNA fragmentation factor 45 kDa subunit, DFF-45, Inhibitor of CAD, ICAD
Gene Name Dffa
Gene ID 13347
Accession Number NP_001020467
Sequence Location Cytoplasm
Biological Function Inhibitor of the caspase-activated DNase (DFF40).
Research Areas Apoptosis
Background A human DNA fragmentation factor (DFF) that is cleaved by caspase-3 during apoptosis was identified recently. The mouse homologue of human DFF was identified as a DNase inhibitor and was designated ICAD (inhibitor of caspase- activated DNase). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in nuclei. Therefore, cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is a hallmark of apoptotic cell death.
Application Images
Description Antibodies: DR4 1167 (4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.
Description Immunohistochemistry of ICAD in mouse lung tissue with ICAD antibody at 2 ug/mL.
Description Immunofluorescence of ICAD in Mouse Lung cells with ICAD antibody at 10 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting : use at 1ug/mL.

Immunohistochemistry: use at 2ug/mL.

Positive control: Mouse lung tissue lysate.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
References & Data Sheet