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Anti-HTLV-1 Tax Antibody (90016)

$520.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ELISA Transfected Cells
SKU: 90016 Categories: , ,
Overview
Product Name Anti-HTLV-1 Tax Antibody (90016)
Description Anti-HTLV-1 Tax Mouse Monoclonal Antibody
Target HTLV-1 Tax
Species Reactivity Transfected Cells
Applications WB,IHC,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 1A3
Isotype IgG2a
Immunogen Recombinant full-length HTLV- Tax protein.
Properties
Form Lyophilized
Formulation Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
Buffer Formulation Tris Buffer
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody reacts with HTLV-1 Tax expressed in transiently transfected cells.
Target Name Protein Tax-1
Target ID HTLV-1 Tax
Uniprot ID P03409
Alternative Names Protein X-LOR, Protein PX, Trans-activating transcriptional regulatory protein of HTLV-1
Gene Name Tax
Sequence Location Host nucleus, Host cytoplasm
Biological Function Transcriptional activator that governs the viral transcription from the 5'LTR via the recruitment of dimers of host phosphorylated CREB1. Together they bind cAMP response elements within the viral promoter and mediate high-level viral transcription (PubMed:8970957). Increases host CREB1 O-GlcNAcylation to further increase 5'LTR transactivation (PubMed:28742148). Modulates also the expression of cellular genes leading to the deregulation of T-cell proliferation, perturbing the integrity of cell cycle checkpoints, the DNA damage response and apopototic pathways. Acts as an ubiquitin E3 ligase and stimulates host IKK complex by catalyzing the assembly of free mixed-linkage polyubiquitin chains, resulting in constitutive activation of the transcription factor NF-kappa-B (PubMed:28103322, PubMed:27082114). Inhibits the host nonsense-mediated mRNA decay (NMD), a cellular process that can actively degrade mRNAs by interacting with host UPF1 (PubMed:27082114). {PubMed:12052856, PubMed:15466468, PubMed:16809310, PubMed:27082114, PubMed:2768259, PubMed:28103322, PubMed:28742148, PubMed:29382845, PubMed:2990037, PubMed:8970957}.
Research Areas Infectious Disease
Background The nonstructural regulatory Tax proteins are unique characteristics of delta retroviruses, a subgroup of retroviruses, which confers long-term persistent infection to mammalian lymphocytes. These proteins are crucial for productive viral replication, and they stimulate the proliferation of host lymphocytic cells. Tax has been shown to be oncogenic because it transforms and immortalizes rodent fibroblasts and human T-lymphocytes. Tax also stimulates cell growth by direct binding to cyclin-dependent kinase holenzymes and/or inactivating tumor suppressors (e.g. p53, DLG). Moreover, Tax silences cellular checkpoints, which guard against DNA structural damage and chromosomal missegregation, thereby favoring the manifestation of a mutator phenotype in cells.
Application Images
Additional Information
Handling
Storage This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
Dilution Instructions Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.
Application Instructions ELISA: use at 0.1-1.0ug/ml

Immunoblotting:

User should use at 2-10ug/ml. A band of ~42kDa is detected.

Immunohistochemistry: use at 2- 20ug/ml.

These are recommended concentrations.

End user should determine optimal concentrations for their applications.
References & Data Sheet