Anti-Hsp90 alpha Monoclonal Antibody
Catalog No.: 11102 (clone D7alpha)
Size: 100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
Hsp90 and the 94 kDa glucose-regulated protein, Grp94, are major molecular chapeones of the cytosol and endoplasmic reticulum. In mammalian cells, there are at least two Hsp90 isoforms, Hsp90α and Hsp90β, which are encoded by separate genes. All known members of the Hsp90 family are highly conserved, especially in the N-terminal and C-terminal regions. In the absence of stress, Hsp90 is an essential component of cellular processes such as hormone signaling and cell cycle control. Several regulatory proteins such as steroid receptors, cell cycle kinases and p53 have been identified as substrates of Hsp90.
Antigen: Full-length Hsp90 purified from chicken brain
Host Species: Mouse
Antibody Class: IgG1
Preservatives: 0.09% sodium azide
Other Additives: 50% glycerol
This antibody recognizes human, mouse, rat, rabbit, bovine, porcine, and chicken Hsp90α (90 kDa).
Immunoblotting: use at 1-5ug/ml. A band of ~90 kDa is detected.
Immunoprecipitation: 5ug on 20ul Protein A-Sepharose + 100ul sample.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Heat-shocked HeLa cell lysate.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
Schuh S et al. 1985 J Biol Chem 260: 14292-14296;
Sullivan WP et al. 1985 Biochemistry 24: 4214-4222.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.