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Anti-Hsp27 Antibody (11120)

$336.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ELISA,IHC,IP Human
SKU: 11120 Categories: , ,
Overview
Product Name Anti-Hsp27 Antibody (11120)
Description Anti-Hsp27 Mouse Monoclonal Antibody
Target Hsp27
Species Reactivity Human
Applications WB,ELISA,IHC,IP
Host Mouse
Clonality Monoclonal
Clone ID 5D12.A12
Isotype IgG2b
Immunogen Human Hsp27
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human Hsp27.
Target Name Heat shock protein β-1
Target ID Hsp27
Uniprot ID P04792
Alternative Names HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27
Gene Name HSPB1
Gene ID 3315
Accession Number NP_001531.1
Sequence Location Cytoplasm, Nucleus, Cytoplasm, cytoskeleton, spindle
Biological Function Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742). {PubMed:10383393, PubMed:19166925, PubMed:20178975, PubMed:23728742}.
Research Areas Heat Shock& Stress Proteins
Background Hsp27 is an important heat shock protein found in normal and malignant human cells. The basic structure of most Hsps is a highly conserved amino acid sequence with an alpha-crystallin domain at the C-terminus and WD/EPF domain at the less conserved N- terminus. The N-terminus is essential for formation of high molecular weight oligomers. Hsp27 oligomers are formed by as many as 8-40 Hsp 27 monomers. The degree of oligomerization is associated with chaperone activity: large oligomers have high chaperone activity, whereas dimers have no chaperone activity. Hsp27 is localized in the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress where it may function to stabilize DNA and/or the nuclear membrane. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with activation of cytochrome C / Apaf-1 / dATP complex, thereby inhibiting activation of procaspase-9.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-1ug/ml. A band of 27 kDa is detected

ELISA: use at 1ug/ml.

Immunohistochemistry: use at 1- 10ug/ml.

Immunoprecipitation: use at 1-10ug/ml.

Positive control: HeLa cell lysate
References & Data Sheet