Anti-Hsp25/Hsp27 Antibody (11110)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF,IP,FCM,FACS Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Hamster
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Overview
Product Name Anti-Hsp25/Hsp27 Antibody (11110)
Description Anti-Hsp25/Hsp27 clone 8A7 Mouse Monoclonal Antibody
Target Hsp25/Hsp27
Species Reactivity Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Hamster
Applications WB,IHC,ICC/IF,IP,FCM,FACS
Host Mouse
Clonality Monoclonal
Clone ID 8A7
Isotype IgG1
Immunogen Hsp27 peptide
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, rat, bovine, canine, guinea pig, and hamster Hsp 25/27. Some cross- reactivity with alpha B crystallin.
Target Name Heat shock protein β-1
Target ID Hsp25/Hsp27
Uniprot ID P04792
Alternative Names HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27
Gene Name HSPB1
Gene ID 3315
Accession Number NP_001532.1
Sequence Location Cytoplasm, Nucleus, Cytoplasm, cytoskeleton, spindle
Biological Function Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742). {PubMed:10383393, PubMed:19166925, PubMed:20178975, PubMed:23728742}.
Research Areas Heat Shock& Stress Proteins
Background Hsp25 is the mouse homologue of human Hsp27, a member of the small heat shock protein family comprised of proteins of ~15->30kDa. Oligomers of Hsp27 consist of as many as 8-40 Hsp27 monomers; large oligomers have high chaperone activity whereas dimers have no chaperone activity. Hsp27 is localized to the cytoplasm of unstressed cells but redistributes to the nucleus in response to stress where it may stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli, and, therefore, has been suggested as an important intermediate in second messenger- mediated signaling pathways. Hsp27 appears to be involved in cell differentiation and may play a role in termination of cell growth.
Application Images
Description Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (11110). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (11110) at 1:5000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.
Description Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (11110). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (11110) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.
Description Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (11110). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (11110) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.
Description Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (11110). Tissue: embryo somites. Species: Rat. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (11110) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Investigation at 11 days gestation. Courtesy of: Mike Welsh, Umich.
Description Western Blot analysis of Rat Lung tissue lysates showing detection of Hsp27 protein using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (11110). Load: 15 µg. Block: 5% blocking solution. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (11110) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse: HRP for 1 hour at RT.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.2ug/mL (ECL). A band of 25-27 kDa is detected.

Immunofluorescence: use at 5ug/mL.

Positive control: HeLa cell lysate
References & Data Sheet