Anti-HSF2 Antibody (11105)

$400.00

Host Quantity Applications Species Reactivity Data Sheet  
Rat 100ug WB,ICC/IF,AM Human, Mouse, Rat, Rabbit, Bovine, Canine, Porcine, Guinea Pig, Hamster, Sheep, Monkey
SKU: 11105 Categories: , ,
Overview
Product Name Anti-HSF2 Antibody (11105)
Description Anti-HSF2 Rat Monoclonal Antibody
Target HSF2
Species Reactivity Human, Mouse, Rat, Rabbit, Bovine, Canine, Porcine, Guinea Pig, Hamster, Sheep, Monkey
Applications WB,ICC/IF,AM
Host Rat
Clonality Monoclonal
Clone ID 3-E2
Isotype IgG1
Immunogen Recombinant full-length mouse HSF2
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes HSF2 in heat- shocked cell lysates of human, mouse, rat, rabbit, bovine, canine, porcine, guinea pig, hamster, sheep, and monkey.
Target Name Heat shock factor protein 2
Target ID HSF2
Uniprot ID P38533
Gene ID 15500
Accession Number NP_001129036.1
Sequence Location Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth and moves to the nucleus upon activation.
Biological Function DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. HSF2 is expressed in a form that binds DNA constitutively but loses DNA binding by incubation at greater than 41 degrees C.
Research Areas Heat Shock& Stress Proteins
Background Heat shock factor 1 (HSF1) is a heat shock transcription factor that activates the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. Up- regulation of expression of heat shock proteins in response to stress occurs at the level of transcription through a heat shock element and a HSF transcription factor. Amino acid sequences for most HSFs are highly conserved. A DNA binding domain is at the N-terminus, hydrophobic repeats (essential to the formation of active trimers) are adjacent to this binding domain, and another short hydrophobic repeat (necessary for suppression of trimerization) occurs toward the C-terminus. HSF2 exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form. Various experiments have suggested that HSF2 may play roles in differentiation and development.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 (11105). Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody (11105) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Description Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 (11105). Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody (11105) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Description Western Blot analysis of Human K562 cell lysates showing detection of HSF2 protein using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 (11105). Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody (11105) at 1:1000. Cells transiently transfected with control, HSF1 or HSF2 shRNA constructs. Courtesy of: Lea Sistonen, Abo Akademi University, Finland.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-5ug/mL. A band of ~69 kDa is detected.Immunohistochemistry / Immuno- fluorescence: use at 1-5 ug/mL.

Immunoprecipitation: use 1-2ug per 100- 500ug total protein.

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: Heat-shocked HeLa cell lysate, K562 cell nuclear extract, human pancreatic tumor cells.
References & Data Sheet