Anti-Hsc70/Hsp73 Monoclonal Antibody
Catalog No.: 11119 (clone 1F2-H5)
Size: 100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
Hsp70 genes, members of a multigene family, encode heat-inducible 70kDa heat- shock proteins that function as molecular chaperones. They have been identified in most organelles of eukaryotic cells as well as in bacteria. The N-terminus of Hsp70 binds ATP with high affinity and the C- terminus binds proteins and polypeptides. When cells are subjected to metabolic stress, Hsp70 is expressed. Hsp70 shares >90% sequence homology with Hsc70. Constitutively expressed Hsc70 forms a stable complex with the highly inducible Hsp70 in cells following heat shock. This interaction is regulated by ATP. Research on Hsc70 suggests that it plays a role in facilitating the recovery of centrosomal structure and function after heat shock.
Antigen: Human Hsc70
Host Species: Mouse
Antibody Class: IgG2a
Preservatives: None. Available on request.
This antibody recognizes human, mouse, and rat Hsc70. Other species have not been tested. It does not cross-react with Hsp70.
Immunoblotting: use at 1ug/ml. A band of ~73kDa is detected.
ELISA: use at 1-10ug/ml Immunohistochemistry: use at 1-10ug/ml. Immunoprecipitation: 1-10ug/ml.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: HeLa cell lysate.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.