HNF4 alpha (Phospho-Ser304) Polyclonal Antibody
Catalog No.: 43043
Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
Hepatocyte nuclear factor 4 alpha (HNF4α) is a nuclear transcription factor that binds DNA as a homodimer and controls the expression of several genes including hepatocyte nuclear factor 1, a transcripton factor that regulates expression of several hepatic genes. HNF4α has been implicated in development of liver, kidney, and intestines. Mutations in the HNF4α gene have been associated with monogenic autosomal-dominant non-insulin-dependent diabetes mellitus type 1. Alternative splicing of this gene results in multiple transcript variants.
Antigen: Peptide sequence that includes phosphorylation site of serine 304 (L-R-S(p)-Q-V) derived from human HNF4α and conjugated to KLH.
Accession no.: P41235, NP_000448.3
Host Species: Rabbit
Specificity: This antibody detects endogenous human, mouse, and rat HNF4α only when phosphorylated at serine 304.
Immunoblotting: use at dilution of 1:1,000. A band of ~52kDa is detected.
These are recommended working dilutions. Enduser should determine optimal dilutions for their applications
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Can be stored at 4°C for short-term use.
For in vitro investigational use only. Not intended for therapeutic or diagnostic applications.