Anti-CCR5 (NT) Antibody
Catalog No.: 1112
Size: 100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chroma-tography.
Human immunodeficiency virus (HIV) and related viruses require coreceptors in addition to CD4 to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b, CCR8, GPR15, Bonzo, GPR1, and V28, have been identified as HIV coreceptors. Among them, CCR5 is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains. CCR5 is required for infection by HIV-1, HIV-2, and SIV. The β-chemokines RANTES, MIP-1α, and MIP-1β are the ligands for CCR5 and prevent infection by macrophage-tropic HIV-1. CCR5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV binding sites. Messenger RNA for CCR5 is expressed in lymphoid cells and tissues.
Antigen: Peptide corresponding to aa 6-20 of human CCR5.
Host Species: Rabbit
Preservatives: 0.02% sodium azide.
This antibody recognizes human CCR5 (40 kD).
Immunoblotting: use at 1:1,000-1:2,000 dilution.
Positive control: Whole cell lysate from THP-1 cells.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.