HIF-1α Monoclonal Antibody
Catalog No.: 34039 (clone ESEE122)
Format: 100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.
Purified by Protein G affinity chromatography.
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor that regulates the transcription of a broad range of genes that facilitate responses to hypoxia including genes that regulate angiogenesis, erythropoiesis, cell cycle, metabolism, and apoptosis. HIF-1 is comprised of two subunits, HIF-1α and HIF-1β. The widely expressed HIF-1α is degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH)-E3 ligase complex; ubiquitination and proteasomal degradation follows.
Antigen: Recombinant protein corresponding to aa 329-530 of human HIF-1α.
Accession no.: Q61221, NP_034561.2
Host Species: Mouse
Antibody Class: IgG1
Specificity: This antibody recognizes human, mouse, rat, and bovine HIF-1α.
Immunoblotting: use at 2ug/ml. Predicted molecular weight ~116kDa.
This is a recommended concentration. Endusers should determine optimal concentrations for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not intended for use in therapeutic or diagnostic procedures.