HER2 (Phospho-Tyr1221/Tyr1222) Polyclonal Antibody
Catalog No.: 43076
Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
HER2 is a member of the human epidermal growth factor receptor (HER/EGFR/ERBB) family. Amplification or over-expression of this oncogene has been shown to play an important role in the development and progression of certain aggressive types of breast cancer. In recent years HER2 has become an important biomarker and target of therapy for approximately 30% of breast cancer patients.
Antigen: Peptide sequence that includes phosphorylation site of tyrosine 1221/1222 (N-L-Y(p)- Y(p)-W) derived from human HER2 and conjugated to KLH.
Host Species: Rabbit
Specificity: This antibody detects endogenous human HER2 only when phosphorylated at tyrosine 1221/1222.
Accession no.: P04626, NP_001005862.1
Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~185kDa is detected.
Immunofluorescence: use at dilution of 1:100-1:200.
These are recommended working dilutions. Endusers should determine optimal dilutions for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Can be stored at 4°C for short-term.
For in vitro investigational use only. Not intended for therapeutic or diagnostic applications.