Anti-Her2/Neu Antibody (34061)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ul IHC Human
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Overview
Product Name Anti-Her2/Neu Antibody (34061)
Description Anti-Her2/Neu Mouse Monoclonal Antibody
Target Her2/Neu
Species Reactivity Human
Applications IHC
Host Mouse
Clonality Monoclonal
Clone ID G002.1
Isotype IgG2a
Immunogen Recombinant human Her2/Neu.
Properties
Form Liquid
Concentration Lot Specific
Formulation Tris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide.
Buffer Formulation Tris
Buffer pH pH 7.3-7.7
Buffer Anti-Microbial 0.1% Sodium Azide
Buffer Protein Stabilizer 1% Bovine Serum Albumin
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity Human Her2/Neu. Reactivity with other species has not been investigated.
Target Name Receptor tyrosine-protein kinase erbB-2
Target ID Her2/Neu
Uniprot ID P04626
Alternative Names EC 2.7.10.1, Metastatic lymph node gene 19 protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, CD antigen CD340
Gene Name ERBB2
Sequence Location [Isoform 1]: Cell membrane, Cytoplasm, perinuclear region. Nucleus. Note=Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. Also detected in VPS35-positive endosome-to-TGN retrograde vesicles (PubMed:31138794).
Biological Function Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. {PubMed:10358079, PubMed:15380516, PubMed:21555369}.
Research Areas Cancer research
Background The Her2/Neu (c-erB-2) proto-oncogene product is a trans-membrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the Her2/Neu protein has been associated with ductal breast cancer as well as with pulmonary and gastric adenocarcinomas. A correlation between Her2/Neu and p53 has also been reported as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer.
Application Images
Description Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes. Detection of Her2/Neu in human breast carcinoma with #34061 diluted 1:100-1:200.
Additional Information
Handling
Storage Store at 2-8°C. Do not freeze.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions

Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.
References & Data Sheet