Mikrogen Diagnostik recomWell Helicobacter pylori IgG ELISA Kit

$393.00

Host Quantity Applications Species Reactivity Data Sheet  
96 Tests ELISA Helicobacter pylori
SKU: 4704 Categories: ,
Overview
Product Name Mikrogen Diagnostik recomWell Helicobacter pylori IgG ELISA Kit (4704)
Description Enzyme immunoassay with antigens produced by recombinant techniques for the detection of IgG and IgA antibodies against Helicobacter pylori

Product is intended for research use.
Target Human Helicobacter pylori IgG
Product Background The gram-negative, spiral-shaped bacterium Helicobacter pylori was described by Warren and Marschall in 1983. The connection between chronic Type B gastritis and Helicobacter pylori has been well studied. In fact, there is a causal relation between Helicobacter pylori and almost all duodenal and stomach ulcers. Moreover, Helicobacter pylori is associated with an increased risk of gastric adenocarcinomas. Apart from strains of different virulence, host factors may also be responsible for this phenomenon.An infection with Helicobacter pylori induces a specific immune response. As a rule, antibodies of the classes IgG and IgA can be detected. Only small amounts of IgM antibodies are identified. Indeed, there is a decrease in the antibody concentration after successful eradication through combined antibiotic therapy.The eradication of Helicobacter pylori cures not only gastritis or peptic ulcer, but also low-malignant gastric MALT lymphomas. Although peptic ulcers are also cured without eradication of Helicobacter pylori, there is a recurrence within one year in up to half of all the cases in the absence of permanent acid suppression treatment. The rate of recurrence is less than 5 % per year after successful microbial eradication.
Species Reactivity Helicobacter pylori
Assay Type Indirect ELISA
Applications ELISA
Properties
Background The gram-negative, spiral-shaped bacterium Helicobacter pylori was described by Warren and Marschall in 1983. The connection between chronic Type B gastritis and Helicobacter pylori has been well studied. In fact, there is a causal relation between Helicobacter pylori and almost all duodenal and stomach ulcers. Moreover, Helicobacter pylori is associated with an increased risk of gastric adenocarcinomas. Apart from strains of different virulence, host factors may also be responsible for this phenomenon.An infection with Helicobacter pylori induces a specific immune response. As a rule, antibodies of the classes IgG and IgA can be detected. Only small amounts of IgM antibodies are identified. Indeed, there is a decrease in the antibody concentration after successful eradication through combined antibiotic therapy.The eradication of Helicobacter pylori cures not only gastritis or peptic ulcer, but also low-malignant gastric MALT lymphomas. Although peptic ulcers are also cured without eradication of Helicobacter pylori, there is a recurrence within one year in up to half of all the cases in the absence of permanent acid suppression treatment. The rate of recurrence is less than 5 % per year after successful microbial eradication.
Sample Type Serum, plasma, whole blood
Assay Resolution See Figure
Components
10X Wash Buffer100 mL
Dilution Buffer125 mL
TMB Substrate12 mL
Stop Slution12 mL
Instuctions for Use1 Each
Evaluation Form1 Each
Microplate Sealing Tape2 Each
Microtiter Plate12 x 8 Wells
Positive Control450 uL
Cut-Off Control450 uL
Negative Control450 uL
Anti-Human IgG Conjugate500 uL
Specificity Information
Target ID Human Helicobacter pylori IgG
Research Areas Infectious Disease
Application Images
Description Test principle and procedure:

Indirect sandwich test. Recombinant antigens are bound to the solid phase.

1. 1st Incubation. Add patient samples diluted 1:101 (sample: 10 µl of serum or plasma), incubate for 1 h at 37 °C.
2. Wash 4 times
3. 2nd Incubation
4. Add peroxidase conjugated anti-human IgG or IgM antibodies (conjugate), incubate for 30 min at 37 °C.
5. Wash 4 times
6. Color reaction
7. Add ready-to-use TMB solution and incubate for 30 min at room temperature. Stop the substrate reaction with H3PO4 and measure the extinction at 450 nm.
Description Specificity
Description Sensitivity
Description Accuracy
Additional Information
Handling
Storage Store at 2 - 8°C. Do not Freeze.
References & Data Sheet