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Anti-HDEL Antibody (11077)

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF Drosophila, Yeast, Plants
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SKU: 11077 Categories: , ,
Overview
Product Name Anti-HDEL Antibody (11077)
Description Anti-HDEL Mouse Monoclonal Antibody
Target HDEL
Species Reactivity Drosophila, Yeast, Plants
Applications WB,ICC/IF
Host Mouse
Clonality Monoclonal
Clone ID 2-E7
Isotype IgG2b
Immunogen Synthetic peptide corresponding to the C-terminus of yeast Bip.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes a ~70 kDa protein corresponding to the predicted molecular mass of HDEL in Drosophila, yeast, and various plants (barnyard grass, beet, cotton, mung bean, sorghum, and wheat).
Target ID HDEL
Research Areas Heat Shock& Stress Proteins
Background The Hsp70 family includes four highly conserved proteins (Hsp70, Hsc70, Grp75, and Grp78) which function as molecular chaperones, in translocation of polypeptides across cell membranes and to cell nuclei, and in proper folding of nascent polypeptide chains. Sorting of proteins by the Hsp70 family relies on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S. cerevisiae.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-1ug/ml. A band of ~70 kDa is detected.

Immunofluorescence: use at 0.2-2ug/ml.

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: S. cerevisiae lysate.
References & Data Sheet
References Napier RM et al. 1992 J Cell Science 102: 261-271.
PMID 1383243