Recombinant non-structural protein 4 (NS4), HCV (sub-type 1B)
Catalog No.: 94304
Size: 1.5 ml
Recombinant Hepatitis C virus NS4 is expressed in E. coli as a monomeric, non-glycosylated polypeptide chain consisting of 252 amino acids with a molecular mass of 26300 Da. The product represents amino acids 1617 to 1864 of the HCV polyprotein, covering the C-terminal end (45 a.a.) of NS3, the complete NS4A sequence (54 a.a.) and the major part of NS4B (153 a.a. of the N-terminus).
Source: Genetically engineered Escherichia coli
Diluent: Antigen specific solution, contains 0.1% SDS. Antigen lots in PBS / 0.1% SDS are prepared on request only.
Purification: Ion Exchange Chromatography
Purity: >98%, analysed by SDS-PAGE (reducing and non-reducing) followed by Coomassie-Blue staining and immunoprobing of Western blotted proteins with anti-E.coli serum (DAKO).
Endotoxin: Not determined
Protein concentration: Determined spectrophotometrically at 280nm (ext. coeff. 32410) and densitometrically by Advanced Image Data Analyzer software (Raytest) in relation to BSA standards.
The integrity of the plasmid is controlled by restriction analysis and DNA sequencing. The expression product is characterized by N-terminal protein sequencing, SDS-PAGE (app. m.w.) and immunodetection of the Western blotted protein. In addition, correct reactivities compared to previous antigen lots with defined human and animal sera (negative, low, medium and high positive) are mandatory.
STORAGE AND SHELF LIFE
At -80°C 10 years from the date of production. Avoid repeated freeze-thaw cycles.
For in vitro investigational use only. Not intended for diagnostic or therapeutic procedures.