Anti-HCN3 Cyclic Nucleotide-Gated Channel Monoclonal Antibody
Catalog No.: 11508 (clone S141-28)
Size: 100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Hyperpolarization-activated cation channels of the HCN gene family, such as HCN3, play a crucial role in the regulation of cell excitability. They contribute to spontaneous rhythmic activity in the heart and brain.
Antigen: Fusion protein aa 660-779 (cytoplasmic C-terminus) of mouse HCN3 (accession no. O88705).
Host Species: Mouse
Antibody Class: IgG1
This antibody recognizes mouse and rat HCN3. It does not cross-react with other HCNs.
Immunoblotting: use at 1-10ug/ml. A band of ~90kDa is detected.
Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml
Immunofluorescence: use at 1-10ug/ml
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat brain lysate
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.