HA HRP

Hemagglutinin (HA) Affinity-Purified Polyclonal Antibody, HRP Conjugate

ORDERING INFORMATION

Catalog No., Size
18850P, 100ug
Format: 1mg/ml conjugated to horseradish peroxidase (HRP) in Phosphate Buffered Saline (PBS), pH 7.2, 0.2% BSA, 0.1% Pro-Clean 400. Molar enzyme/antibody ratio = 4:1.

BACKGROUND

The HA epitope tag is derived from the HA-molecule corresponding to amino acids YPYDVPDYA of human influenza hemagglutinin. It is frequently used as an epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.

SPECIFICATION SUMMARY

Antigen: HA (YPYDVPDYA) conjugated to KLH.
Host Species: Rabbit
Specificity: Antibody was affinity-purified on the peptide.

APPLICATIONS

Immunoblotting: use at a dilution of 1:1,000- 1:30,000.
Immunocytochemistry: use at a dilution of 1:200-1:500.
ELISA: use at a dilution of 1:10,000- 1:100,000.
These are recommended dilutions. Endusers should determine optimal dilutions for their applications.

DILUTION INSTRUCTIONS

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE AND STABILITY

This antibody is stable for at least one (1) year at 2-8°C.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.

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