Anti-HA Antibody, HRP conjugate

$498.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,IHC,ICC,ELISA Ha
SKU: 18850P Categories: , ,
Overview
Product Name Anti-HA Antibody, HRP conjugate (18850P)
Description Anti-HA HRP Rabbit Polyclonal Antibody
Target HA, HRP conjugate
Species Reactivity Ha
Applications WB,IHC,ICC,ELISA
Host Rabbit
Clonality Polyclonal
Isotype Whole IgG
Conjugate HRP
Conjugate Ratio HRP, Molar enzyme/antibody ratio = 4:1.
Immunogen HA (YPYDVPDYA) conjugated to KLH.
Source Escherichia coli
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.1% Pro-Clean 400
Buffer Protein Stabilizer 0.2% Bovine Serum Albumin
Format Purified
Purification Purified by antigen immuno-affinity chromatography
Purity Greater than 95% as determined by SDS-PAGE and RP- HPLC
Specificity Information
Specificity Antibody was affinity-purified on the peptide.
Target ID HA, HRP conjugate
Research Areas Epitope Tags
Background The HA epitope tag is derived from the HA-molecule corresponding to amino acids YPYDVPDYA of human influenza hemagglutinin. It is frequently used as an epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.
Application Images
Description Detection of HA-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, rabbit anti-HA antibody 18850P used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
Additional Information
Handling
Storage Store at 2 - 8°C. Do not Freeze.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000- 1:30,000.

Immunocytochemistry: use at a dilution of 1:200-1:500

ELISA: use at a dilution of 1:10,000- 1:100,000

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
References & Data Sheet