Anti-HA Antibody

$406.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,IP,ICC,ELISA Ha
SKU: 18850-01 Categories: , ,
Overview
Product Name Anti-HA Antibody (18850-01)
Description Anti-HA Rabbit Polyclonal Antibody
Target HA
Species Reactivity Ha
Applications WB,IP,ICC,ELISA
Host Rabbit
Clonality Polyclonal
Immunogen HA (YPYDVPDYA) conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.2, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.09% Sodium Azide
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity Antibody was affinity-purified on the peptide.
Target ID HA
Research Areas Epitope Tags
Background The HA epitope tag is derived from the HA-molecule corresponding to amino acids YPYDVPDYA of human influenza hemagglutinin. It is frequently used as an epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.
Application Images
Description Detection of HA-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein.Antibody: Affinity purified rabbit anti-HA antibody 18850 used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
Additional Information
Handling
Storage Store at 2 - 8ºC. Do not Freeze.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000- 1:20,000.

Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunoprecipitation: use 1-4ug/mg lysate.

ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
References & Data Sheet