Overview
Product Name Guanosine ELISA Kit (ECP7868)
Description Guanosine ELISA Kit (ECP7868) is a Competitive Inhibition ELISA for the quantitiation of Guanosine in a variety of aqueous sample types.
Target Guanosine
Species Reactivity Pan-Species
Assay Type Competitive Inhibition
Applications ELISA
Properties
Molecular Mass 283.24 g·mol−1
Background Minimum Level of Detection: 82.7 pg/mL
Dynamic range of quantitation: 246.9-20,000 pg/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Dynamic range of quantitation: 246.9-20,000 pg/mL
Intra-Assay Reproducibility: <10% CV, where n=20 for 3 sample concentrations at low, medium and high levels of the Dynamic Range within an experimental plate.
Inter-Assay Reproducibility: <12% CV, where n=8 for 3 samples concentrations at low, medium and high levels of the Dynamic Range measured acorss 3 replicate plates.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other aqueous sample types.
Assay Resolution Quantitative
Components
Reagents | Quantity |
Guanosine Well Plate, Ready to Use (96 Wells) | 1 Each (8 x 12 Wells) |
Adhesive Plate Seal | 4 Each |
Guanosine Standard | 2 Vials |
Standard Diluent | 1 x 20mL |
Detection Reagent A | 1 x 120uL |
Diluent A | 1 x 12mL |
Detection Reagent B | 1 x 120uL |
Diluent B | 1 x 12mL |
TMB Substrate | 1 x 9mL |
Stop Solution | 1 x 6mL |
30X Wash Buffer | 1 x 20mL |
Specificity Information
Target ID Guanosine
Alternative Names guanine riboside, vernine, Guanozin
CAS Number 118-00-3
PubChem ID 135398635
Research Areas Biochemicals
Background Guanosine is a purine nucleoside in which guanine is attached to ribofuranose via a beta-N(9)-glycosidic bond. It has a role as a fundamental metabolite. It is a purines D-ribonucleoside and a member of guanosines. It is functionally related to a guanine.
Application Images

Description Typical standard curve
Additional Information
Additional Information Assay Duration: Approximately 2 hours
Handling
Storage 4°C for 1 month. Recommended -20°C for long-term storage until indicated expiration date.