GSK3β (Phospho-Ser9) Polyclonal Antibody
Catalog No.: 43002
Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
GSK3β participates in the Wnt signaling pathway and is implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. GSK3β phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. It also phosphorylates CTNNB1/beta-catenin and MUC1 in breast cancer cells and decreases the interaction of MUC1 with CTNNB1/beta-catenin.
Antigen: Peptide sequence that includes phosphorylation site of serine 9 (T-T-S(p)-F-A) derived from Human GSK3β and conjugated to KLH.
Accession no.: P49841, NP_001139628.1
Host Species: Rabbit
Specificity: This antibody detects endogenous human, mouse, and rat GSK3 beta only when phosphorylated at serine 9.
Western blotting: use at dilution of 1:500- 1:1,000. A band of ~46kDa is detected.
Immunohistochemistry: use at dilution of 1:50- 1:100.
These are recommended working dilutions. Enduser should determine optimal dilutions for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Can be stored at 4°C for short-term.
For in vitro investigational use only. Not intended for therapeutic or diagnostic applications.