GSK3α (Phospho-Ser21) Polyclonal Antibody
Catalog No.: 43007
Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
GSK3 acts as a multifunctional downstream switch that determines the output of numerous signaling pathways. There are two mammalian GSK-3 isoforms encoded by distinct genes, GSK3 alpha and GSK3 beta, which are structurally similar, but functionally non-identical. GSK3α is inhibited by phosphorylation at S21 by Akt and other kinases. Dysregulated GSK3 has been implicated in several diseases including type II diabetes, Alzheimers disease, and cancer.
Antigen: Peptide sequence that includes phosphorylation site of serine 21 (T-S-S(p)-F-A) derived from human GSK3α and conjugated to KLH.
Accession no.: P49840, NP_063937.2
Host Species: Rabbit
Specificity: This antibody detects endogenous human, mouse, and rat GSK3α only when phosphorylated at serine 21 and does not detect GSK3β phosphorylated at Ser9.
Western blotting: use at dilution of 1:500- 1:1,000. A band of ~51kDa is detected.
Immunohistochemistry: use at dilution of 1:50- 1:100.
These are recommended working dilutions. Enduser should determine optimal dilutions for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Can be stored at 4°C for short-term.
For in vitro investigational use only. Not intended for therapeutic or diagnostic applications.