Anti-Glypican-1 Antibody (34029)

$434.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IF Human, Mouse
SKU: 34029 Categories: , ,
Overview
Product Name Anti-Glypican-1 Antibody (34029)
Description Anti-Glypican-1 Mouse Monoclonal Antibody
Target Glypican-1
Species Reactivity Human, Mouse
Applications WB,IF
Host Mouse
Clonality Monoclonal
Clone ID 9-E9
Isotype IgG1
Immunogen Recombinant full-length human glypican-1.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes native and recombinant human and mouse glypican-1.
Target Name Glypican-1 [Cleaved into: Secreted glypican-1]
Target ID Glypican-1
Uniprot ID P35052
Gene Name GPC1
Sequence Location Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Endosome. Note=S-nitrosylated form recycled in endosomes. Localizes to CAV1-containing vesicles close to the cell surface. Cleavage of heparan sulfate side chains takes place mainly in late endosomes. Associates with both forms of PRNP in lipid rafts. Colocalizes with APP in perinuclear compartments and with CP in intracellular compartments. Associates with fibrillar APP amyloid-beta peptides in lipid rafts in Alzheimer disease brains.; [Secreted glypican-1]: Secreted, extracellular space.
Biological Function Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (By similarity). May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. {PubMed:19936054, PubMed:21642435}.
Research Areas Cancer research
Background Glypican-1 is a widely expressed, GPI anchored, cell surface proteoglycan that has been reported to be important in cellular adhesion and migration. Glypican-1 also functions as an accessory molecule for signaling receptors. Glypican-1 is overexpressed in human gliomas and pancreatic ductal cell carcinomas. Expression of glypican-1 is associated with the aggressive behavior of tumor cells in vitro and in vivo.
Application Images
Description Immunoblotting: at 1-10ug/ml, a band of approx. 64 kDa is detected in normal mouse brain.
Description Immunofluorescence: at 1-10ug/ml, positive staining of human pancreatic carcinoma cell line Capan-1.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions ELISA: at 1-5ug/ml will detect recombinant glypican-1 on the solid phase at 200ng/well.

Immunoblotting: at 1-10ug/ml, a band of approx. 64 kDa is detected in normal mouse brain.

Immunofluorescence: at 1-10ug/ml, positive staining of human pancreatic carcinoma cell line Capan-1.

These are recommended concentrations. User should determine optimal concentrations for their applications.
References & Data Sheet