Glypican-1 Monoclonal Antibody
Catalog No.: 34029
Size: 100 ug
Protein G-purified antibody in PBS, pH 7.4.
Antigen: Recombinant full-length human glypican-1.
Clone no.: 9E9
Host Species: Mouse
Antibody Class: IgG1
Preservatives: None. Available on request.
Glypican-1 is a widely expressed, GPI anchored, cell surface proteoglycan that has been reported to be important in cellular adhesion and migration. Glypican-1 also functions as an accessory molecule for signaling receptors. Glypican-1 is overexpressed in human gliomas and pancreatic ductal cell carcinomas. Expression of glypican-1 is associated with the aggressive behavior of tumor cells in vitro and in vivo.
This antibody recognizes native and recombinant human and mouse glypican-1.
ELISA: at 1-5ug/ml will detect recombinant glypican-1 on the solid phase at 200ng/well.
Immunoblotting: at 1-10ug/ml, a band of approx. 64 kDa is detected in normal mouse brain.
Immunofluorescence: at 1-10ug/ml, positive staining of human pancreatic carcinoma cell line Capan-1.
These are recommended concentrations. User should determine optimal concentrations for their applications.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.