Anti-Glutathione-S-Transferase (GST) Antibody, HRP conjugate (18844P)

$400.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,IHC,ICC,ELISA Glutathione-S-Transferase (Gst)
SKU: 18844P Categories: , ,
Overview
Product Name Anti-Glutathione-S-Transferase (GST) Antibody, HRP conjugate (18844P)
Description Anti-Glutathione-S-Transferase (GST) HRP Rabbit Polyclonal Antibody
Target Glutathione-S-Transferase (GST)
Species Reactivity Glutathione-S-Transferase (Gst)
Applications WB,IHC,ICC,ELISA
Host Rabbit
Clonality Polyclonal
Isotype Whole IgG
Conjugate HRP
Conjugate Ratio HRP, Molar enzyme/antibody ratio = 4:1.
Immunogen Glutathione-S-Transferase (GST) from Schistosoma japonicum.
Source Escherichia coli
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.1% Pro-Clean 400
Buffer Protein Stabilizer 0.2% Bovine Serum Albumin
Format Purified
Purification Purified by affinity on GST
Purity Greater than 95% as determined by SDS-PAGE and RP- HPLC
Specificity Information
Specificity Antibody was affinity-purified on GST.
Target Name Glutathione S-transferase class-mu 28 kDa isozyme
Target ID Glutathione-S-Transferase (GST)
Uniprot ID P26624
Biological Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.; GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
Research Areas Epitope Tags
Background A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids which, compared to tags such as the myc-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. Many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
Application Images
Description Detection of GST-tagged Protein by western blot. Samples: 1.0, 0.3, or 0.1 µg of 293 cell lysate expressing a GST-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, rabbit anti-GST antibody 18844P used for WB at 0.2 µg/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 3 minutes.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at 2-8°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000- 1:30,000.

Immunocytochemistry: use at a dilution of 1:200-1:500

ELISA: use at a dilution of 1:10,000- 1:100,000

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
References & Data Sheet