Glutathione Reductase Monoclonal Antibodies
Catalog Nos.: 13901 (clone 2A2-1B7) and 13902 (clone 2A4-1G9)
Format: 200ug/clone in phosphate-buffered saline, pH 7.4
Glutathione reductase, also known as glutathione-disulfide reductase, is an enzyme that in humans is encoded by the GSR gene. Glutathione reductase catalyzes the reduction of glutathione disulfide to the sulfhydryl form glutathione (GSH), which is a critical molecule in resisting oxidative stress and maintaining the reducing environment of the cell. The importance of the glutathione pathway and enzymes that affect it is gaining an increased level of attention. Although glutathione reductase has been an attractive target for many pharmaceuticals, there have been no successful glutathione reductase related therapeutic compounds created to date.
Antigen: Recombinant human glutathione reductase purified from E. coli expressing the human glutathione reductase gene (aa 44-522).
Accession no.: P00390, NP_000628
Host Species: Mouse
Antibody Class: IgG1κ
Specificity: These antibodies recognize human glutathione reductase. They do not cross-react with yeast glutathione reductase.
ELISA: Titration of glutathione reductase MAbs with glutathione reductase at 3ug/ml on the wells.
ELISA Pairs: Both of these antibodies function as capture and detection antibodies at 1ug/ml.
Immunoblotting: use at 0.5-1.0ug/ml. A band of ~57kDa is detected.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
These antibodies are stable for at least one (1) year at -20°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not intended for therapeutic or diagnostic procedures.