NMDAR NR3A/B Antibody
Catalog No.: 60100
Size: 100 ug IgG1 in 100 ul PBS, pH 7.4, purified by Protein G Chromatography
Two new subunits of the NMDA subtype of glutamate receptor (NMDAR) have been identified, NR3A and NR3B, with NR3B likely being the final member of the NMDAR family. These subunits show high sequence homology, although different distribution patterns: NR3B is expressed predominately in motor neurons, while NR3A is more widely distributed. When expressed in Xenopus oocytes, NR3A or NR3B assemble with NR1 to form excitatory glycine receptors that are unaffected by glutatmate.
Immunogen: Fusion protein of NR3A aa sequence 780-914 from the extracellular III- IV loop.
Host Species: Mouse
This antibody recognizes NMDAR subunits NR3A and NR3B. It does not cross-react with subunits NR1 or NR2A-D.
Immunoblotting: Use at 1-10 ug/ml
Western Blot: Use at 1-10 ug/ml Immunoprecipitation
Dilute in PBS or medium that is appropriate for the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Chatterton, et. al. Nature 415: 793-79. (2002)
Wong, et. al., J. Comp. Neurol. 450: 303- 317 (2002)
Perez-Otano, et. al., J. Neurosci. 21: 1228- 1237 (2001)
Sasaki, et. al., J. Neurophysiol. 87: 2052- 2063 (2002)
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.