Anti-GFRα-2 Antibody (1135)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC Human, Mouse, Rat
SKU: 1135 Categories: , ,
Overview
Product Name Anti-GFRα-2 Antibody (1135)
Description Anti-GFR-2 (IN) Rabbit Polyclonal Antibody
Target GFRα-2
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,ICC
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 377- 391 of human GFRa-2. This peptide sequence is identical to those of mouse and rat.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat GFRalpha-2 (52 kD).
Target Name GDNF family receptorα-2
Target ID GFRα-2
Uniprot ID O00451
Alternative Names GDNF receptorα-2, GDNFR-α-2, GFR-α-2, GDNF receptor β, GDNFR-β, Neurturin receptorα, NRTNR-α, NTNR-α, RET ligand 2, TGF-β-related neurotrophic factor receptor 2
Gene Name GFRA2
Gene ID 2675
Accession Number NP_001158510.1
Sequence Location Cell membrane, Lipid-anchor, GPI-anchor
Biological Function Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor.; [Isoform 2]: Participates in NRTN-induced 'Ser-727' phosphorylation of STAT3. {UniProtKB:O08842}.
Research Areas Neuroscience
Background Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN), play key roles in the control of vertebrate neuronal survivial and differentiation. Physiological responses to NTN require the presence of a novel glycosylphosphatidylinositol linked protein, NTNRalpha, which is a cell surface receptor for NTN. The cDNAs encoding NTNRalpha from human, rat, chicken, and mouse have been cloned. NTNRalpha has also been referred to as GFRalpha-2, GDNFRbeta, Ret ligand 2 (RETL2), and TGF-beta-related neurotrophic factor receptor 2 (TmR2). GFRalpha-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus, NTN, GFRalpha-2, and the Ret PTK form a complex to transmit the NTN signal and to mediate NTN function.
Application Images
Description Western blot analysis of GFR alpha 2 in HeLa total cell lysate with GFR alpha 2 antibody at 1 ug/mL.
Description Immunocytochemistry of GFR alpha 2 in HeLa cells with GFR alpha 2 antibody at 5 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:1,000-1:2,000 dilution.

Positive control: Whole cell lysate of HeLa cells.
References & Data Sheet