Anti-GFRα-1 Antibody (1133)

$469.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Human, Mouse, Rat
SKU: 1133 Categories: , ,
Overview
Product Name Anti-GFRα-1 Antibody (1133)
Description Anti-GFR-1 (IN) Rabbit Polyclonal Antibody
Target GFRα-1
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 369- 382 of human GFRa-1. This peptide sequence is identical to those of mouse and rat.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat GFRalpha-1 (approx. 50 kD).
Target Name GDNF family receptorα-1
Target ID GFRα-1
Uniprot ID P56159
Alternative Names GDNF receptorα-1, GDNFR-α-1, GFR-α-1, RET ligand 1, TGF-β-related neurotrophic factor receptor 1
Gene Name GFRA1
Gene ID 2674
Accession Number NP_001138925.1
Sequence Location Cell membrane, Golgi apparatus, trans-Golgi network, Endosome, Endosome, multivesicular body
Biological Function Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor (By similarity). {ECO:0000250}.
Research Areas Neuroscience
Background Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of the kidney and the enteric nervous system. Physiological responses to GDNF require the presence of a novel glycosylphosphatidylinositol linked protein, GDNFRalpha, a cell surface receptor for GDNF. The cDNAs encoding GDNFRalpha from human, rat, chicken, and mouse have been cloned. GDNFRalpha has also been referred to as GFRalpha-1, Ret ligand 1 (RETL1) and TGF- beta-related neurotrophic factor receptor 1 (TmR1). GFRalpha-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFRalpha, and the Ret PTK form a complex to transmit the GDNF signal and to mediate GDNF function. GFRalpha-1 has been identified in brain, liver, kidney, and spleen.
Application Images
Description Western blot analysis of GFR alpha 1 in crude membrane fractions of human brain (B), liver (L), kidney (K), and spleen (S), respectively, with GFR alpha 1 antibody at 1 ug/mL.
Description Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 2.5 ug/ml.
Description Immunofluorescence of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 20 ug/mL. Red: GFR alpha 1 Antibody (1133)
Blue: DAPI staining
Description Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 1 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500 dilution.
References & Data Sheet