Anti-GABA Receptor Associated Protein Antibody (56300)

Host	Quantity	Applications	Species Reactivity	Data Sheet
Rabbit	100ug	WB,ELISA	Human

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Overview

Product Name Anti-GABA Receptor Associated Protein Antibody (56300)

Description Anti-GABARAP Rabbit Polyclonal Antibody

Target GABA Receptor Associated Protein

Species Reactivity Human

Applications WB,ELISA

Host Rabbit

Clonality Polyclonal

Immunogen Peptide derived from the N- terminus of GABARAP.

Properties

Form Liquid

Concentration Lot Specific

Formulation PBS.

Buffer Formulation Phosphate Buffered Saline

Format Purified

Purification Purified by immunoaffinity chromatography

Specificity This antibody recognizes human GABARAP (14 kD).

Target Name γ-aminobutyric acid receptor-associated protein

Target ID GABA Receptor Associated Protein

Uniprot ID O95166

Alternative Names GABA(A receptor-associated protein, MM46

Gene Name GABARAP

Sequence Location Cytoplasmic vesicle, autophagosome membrane, Endomembrane system, Cytoplasm, cytoskeleton, Golgi apparatus membrane, Cytoplasmic vesicle

Biological Function Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton (PubMed:9892355). Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:15169837, PubMed:20562859, PubMed:22948227). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538). Also required for the local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction (PubMed:25684205). Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation (PubMed:25684205). Involved in apoptosis (PubMed:15977068). {PubMed:15169837, PubMed:15977068, PubMed:20562859, PubMed:22948227, PubMed:25684205, PubMed:31006538, PubMed:9892355}.

Research Areas Neuroscience

Background Type-A receptors for the neurotransmitter GABA (gamma- aminobutyric acid) are ligand-gated chloride channels that mediate inhibitory neurotransmission. A cellular protein, GABA(A)-receptor associated protein (GABARAP), that interacts with the gamma 2 subunit of GABA(A) receptors and modulates receptor channel kinetics was recently identified. GABARAP binds to GABA(A) receptors in vitro and in vivo. Sequence analysis shows similarity between GABARAP and light chain-3 of microtubule-associated proteins 1A and 1B. The interactions between GABA(A) receptors, GABARAP and tubulin suggest a mechanism for the targeting and clustering of GABA(A) receptors.

Handling

Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.

Application Instructions Immunoblotting: use at 1:500-1:1,000 dilution

ELISA: use at 1:1,000-1:5,000 dilution.

Data Sheet