Francisella tularensis LPS Monoclonal Antibodies
Catalog Nos.: 18741 (clone T14) and 18742 (clone FB11)
Size: 200ug Protein G-purified antibody in PBS, pH 7.4., 0.1% sodium azide
Francisella tularensis is a small, nonmotile, aerobic, gram-negative coccobacillus and the etiologic agent of tularemia. It has a thin lipopolysaccharide-containing envelope and is a resilient non-spore-forming bacterium that can survive for several weeks at low temperatures in water, soil, hay, and decaying animal carcasses. Lipopolysaccharide (LPS) is a major species-specific antigen of Fr. tularensis.
Antigen: #18741 (T14) was derived from mice immunized with pooled water- soluble antigens from disintegrated microbial mass of Fr. tularensis vaccine strain. #18742 (FB11) was derived from mice immunized with whole Fr. tularensis.
Host Species: Mouse
Antibody Class: #18741 (T14) = IgG3; #18742 (FB11) = IgG2a
STORAGE AND STABILITY
These antibodies are stable for at least one (1) year at -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.
These antibodies specifically recognize Fr. tularensis LPS. They do not cross- react with Fr. novicida, B. abortus, B. suis, B. melitensis, B. ovis, B. neotomae, Y. pestis, Y. pseudotuberculosis, Y. enterocolitica, S. typhimurium, V. cholera, or E. coli.
The epitope for #18742 (FB11) is located on the O-antigen polysaccharide chain of Fr. tularensis LPS which consists of tetrasaccharide fragments.
Tetrasaccharide D-GalpNAcAN-(1-4)- alpha-D-GalpNAcAN-(1-3)-beta-D- QuipNAc-(1-2)-beta-D-Quip4NFm and trisaccharide D-GalpNAcAN-(1-3)-beta- D-QuipNAc-(1-2)-beta-D-Quip4NFm compete with LPS of Fr. tularensis in ELISA for binding MAb #18742 (FB11).
The epitope for #18741 (T14) has not been identified.
ELISA: use at 1-5ug/ml
Immunofluorescence: use at 5-10ug/ml
These are recommended concentrations; endusers should determine optimal concentrations for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.