Anti-FLIPγ/δ (CT) Antibody
Catalog No.: 2407
Size: 100ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.
Caspase-8 (FLICE) and -10 (FLICE2) are two pivotal members of the ICE/CED-3 protease family. FLICE-inhibitory proteins have been identified in viruses and human cells and are designated v-FLIPs and FLIP, respectively. Human FLIP was cloned by several independent laboratories and designated Casper, I-FLICE, FLAME-1, CASH, and CLARP. FLIP contains two death effector domains and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and -10 and potently inhibits apoptosis induced by death receptors CD95, DR3, TRAIL-R, and TNFR1. Four splice variants of FLIP have been identified and designated FLIP α, β, γ, and δ.
Antigen: Peptide corresponding to aa 191-209 at the C-terminus of human FLIPδ/FLIPS form (accession no. AAC51623).
Host Species: Rabbit
Preservatives: 0.02% sodium azide.
This antibody recognizes human FLIPγ (35kDa) and FLIPδ(25kDa) but not FLIPα or FLIPβ.
Immunoblotting : use at 1ug/ml.
Immunocytochemistry: use at 2ug/ml.
Positive control: Whole cell lysate from HeLa, Jurkat,THP-1, A431, K562, or NIH3T3 cells.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.