Anti-mFLIP (CT) Antibody (2423)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC Mouse
SKU: 2423 Categories: , ,
Overview
Product Name Anti-mFLIP (CT) Antibody (2423)
Description Anti-mFLIP (CT) Rabbit Polyclonal Antibody
Target mFLIP (CT)
Species Reactivity Mouse
Applications ELISA,WB,ICC
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 449-465 at the C-terminus of mouse FLIPL/CASH form (accession no. NP_997536).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes mouse FLIPL form (55kDa) but not FLIPS form.
Target Name CASP8 and FADD-like apoptosis regulator
Target ID mFLIP (CT)
Uniprot ID O15519
Alternative Names Caspase homolog, CASH, Caspase-eight-related protein, Casper, Caspase-like apoptosis regulatory protein, CLARP, Cellular FLICE-like inhibitory protein, c-FLIP, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, I-FLICE, MACH-related inducer of toxicity, MRIT, Usurpin [Cleaved into: CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12]
Gene Name CFLAR
Gene ID 8837
Accession Number NP_001120655.1
Biological Function Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. {PubMed:9880531}.
Research Areas Apoptosis
Background Caspase-8 (FLICE) and -10 (FLICE2) are two pivotal members of the ICE/CED-3 protease family. FLICE-inhibitory proteins have been identified in viruses and human cells and are designated v-FLIPs and FLIP, respectively. Human FLIP was cloned by several independent laboratories and designated Casper, I-FLICE, FLAME-1, CASH, and CLARP. FLIP contains two death effector domains and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and -10 and potently inhibits apoptosis induced by death receptors CD95, DR3, TRAIL-R, and TNFR1. Four splice variants of FLIP have been identified and designated.
Application Images
Description Western blot analysis of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2 ug/ml.
Description Immunocytochemistry of FLIP in 3T3 cells with FLIP antibody at 5 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting : use at 2ug/mL.

Immunocytochemistry: use at 5ug/mL.

Positive control: Whole cell lysate from NIH/3T3 cells.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
References & Data Sheet