Anti-FLASH Antibody (2271)

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF Human, Mouse
Buy Now on Leinco.com
SKU: 2271 Categories: , ,
Overview
Product Name Anti-FLASH Antibody (2271)
Description Anti-FLASH (CT) Rabbit Polyclonal Antibody
Target FLASH
Species Reactivity Human, Mouse
Applications ELISA,WB,ICC,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 1253-1268 of human FLASH (accession no. AF165161). This sequence differs by one amino acid from that of mouse.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human and mouse FLASH (220kDa).
Target Name CASP8-associated protein 2
Target ID FLASH
Uniprot ID Q9UKL3
Alternative Names FLICE-associated huge protein
Gene Name CASP8AP2
Gene ID 9994
Accession Number NP_036247
Sequence Location Cytoplasm. Nucleus. Nucleus, PML body. Mitochondrion. Note=Exported from the nucleus to the mitochondria upon FAS activation.
Biological Function Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase. {PubMed:12477726, PubMed:15698540, PubMed:17003125, PubMed:17245429}.
Research Areas Apoptosis
Background A mammalian homologue of CED-4 has been identified and designated FLASH (FLICE-associated huge protein). FLASH is involved in Fas-induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH, resulting in activation of caspase-8. FLASH is expressed in multiple tissues and cell types.
Application Images
Description Western blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 ug/mL.
Description Immunocytochemistry of FLASH in HeLa cells with FLASH antibody at 10 ug/mL.
Description Immunofluorescence of FLASH in HeLa cells with FLASH antibody at 10 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5ug/mL.

Immunocytochemistry: use at 10ug/mL.

Positive control: HeLa cell lysate.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
References & Data Sheet