FIH (Asparaginyl hydroxylase) Monoclonal Antibody
Catalog No.: 34038 (clone FIH162C)
Format: 100ug in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol. Purified by Protein G affinity chromatography.
FIH, Factor Inhibiting HIF-1 (hypoxia-inducible factor), is an asparaginyl hydroxylase. FIH, in conjunction with VHL, represses HIF-1 transcriptional activity by disrupting the interaction of HIF- 1 with the transcriptional coactivators CBP/p300, and by recruiting histone deacetylases. FIH activity is inhibited during hypoxia. Recent studies show that low nuclear expression of FIH is a prognostic indicator for poor overall survival in cases of clear cell renal cell carcinoma.
Antigen: Recombinant full-length human FIH expressed in E. coli.
Accession no.: Q9NWT6, NP_060372.2
Host Species: Mouse
Antibody Class: IgG1
Specificity: This antibody recognizes human FIH.
Immunoblotting: use at 10ug/ml. A band of ~45kDa is detected.
Immunofluorescence: use at 10ug/ml.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
This antibody is stable for at least one (1) year at -20°C. Avoid repeated freeze-thaw cycles.
For in vitro investigational use only. Not intended for diagnostic or therapeutic procedures.